Signaling Pathway Correlations

How Correlations were Calculated

Spearman rank correlations at the single cell level were calcualted across all cell type-pathway pairs (i.e. HSC source T-cells MHC-I target). Correlations were filtered such that pathways in a pair shared no functional genes. They were then filtered such that their adjusted p-value < 0.05. These correlations were then collapsed to pathway level correlations by averaging their cell type-pathway pair correlations and taking the best adjusted p-value.

As an example, the top 10 positive and negative pathway correlations for HSCs are shown below. Here, the highest positive correlation in HSCs is between HSC CCL source and HSC PARs source (r = 0.736). The highest negative correlations in HSCs is between HSC MHC-I source and HSC PARs target (r = -0.403).

Most Positive Correlated Pair: send MHC-II and send MHC-I

Most Negative Correlated Pair: send MHC-I and send CD52